[R35] DNA Replication and Cytokinesis.
Ente: National Institute of General Medical Sciences
Scadenza: 2028-08-31
Importo max: 26.569 EUR
Paese: US
Descrizione
This is an application for the Administrative Supplement to the currently active R35GM148158 grant entitled
“DNA replication and cytokinesis”. The major thrust of the parent grant is to understand the functions of
replication initiators Orc6 and ORC in eukaryotic cells and tissues using Drosophila and human cells as model
systems. ORC, heteromeric six-subunit protein complex, is essential for DNA replication. The smallest of ORC
subunits, Orc6 protein, is important for DNA replication in all eukaryotes. We have shown that in Drosophila Orc6
has an additional role in cytokinesis through interaction with the septin complex, a highly conserved polymerizing
protein assembly that is critical for cytokinesis in many species and is recognized as important component of the
cytoskeleton. In preliminary studies we have identified structural features of Orc6 that contribute directly to the
functions of the protein in replication and cytokinesis. The overall goal of this proposal is to define the distinct
mechanisms of Orc6 function in DNA replication and in cytokinesis using Drosophila as a model system. All
major objectives of the parent grant require purification of the wild type and mutant protein complexes (such as
ORC and septin complex and their subunits) to study protein functions and protein/DNA interactions. In vivo
analyses in the lab require the use of cell cultures as well as tissues isolated from live Drosophila. To maintain
proteins, cell lines, tissues, DNA constructs etc. it is imperative to utilize reliable storage including -86oC freezer.
Unexpectedly, recently our lab -80oC freezer failed and we currently use departmental 30-year-old -70 oC freezer
that proved to be unreliable, as we had to fix the compressor and various other issues. Therefore, administrative
supplement through PA-20-272 would allow us to replace the broken and obsolete freezer and purchase new
reliable ULT freezer, Eppendorf Cryocube 570h that will greatly help in our studies and workflow. The purchase
of this new equipment required for the project and associated research expenses will provide the necessary
investment into the research-oriented economy.
Istituzione: UNIVERSITY OF ALABAMA AT BIRMINGHAM
PI: IGOR N CHESNOKOV
Progetto: 3R35GM148158-03S1
Settori: National Institute of General Medical Sciences
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