[R21] Characterization of biofilm formation in shigellosis
Ente: National Institute of Allergy and Infectious Diseases
Scadenza: 2028-05-31
Importo max: 236.538 EUR
Paese: US
Descrizione
Abstract
The intestinal pathogen Shigella flexneri is the causative agent of bacillary dysentery and is responsible for
more than 250 million cases of dysentery annually, resulting in more than 200,000 deaths. S. flexneri is an
intracellular pathogen that invade epithelial cells in the colon and spread from cell to cell. The dissemination
process relies on a bacterial adaptor termed IcsA that recruits key components of the actin cytoskeleton and
supports actin-based motility. We have recently discovered that in addition of its intracellular role in
dissemination, IcsA also support bile salt-dependent biofilm formation. Here, we propose to characterize the
structural determinants that support IcsA-mediated bile salts-dependent biofilm formation (Aim1) and the role
of IcsA in extracellular and intracellular colonization in an infant rabbit model of shigellosis (Aim2). The
characterization of the dual functions of IcsA will provide novel insights into the mechanisms supporting
bacillary dysentery in humans.
Istituzione: UNIVERSITY OF VIRGINIA
PI: HERVE F AGAISSE
Progetto: 1R21AI193486-01A1
Settori: National Institute of Allergy and Infectious Diseases
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