[R01] All-optical interrogation of the biological mechanisms of learning

Ente: National Eye Institute

Scadenza: 2030-06-30

Importo max: $740,790

Paese: US

Descrizione

PROJECT SUMMARY The goal of this project is to establish and leverage a new paradigm to optically measure and track synaptic weights in the brains of awake animals as they learn and store information. By combining cellular resolution optogenetic photostimulation and a genetically encoded voltage indicator for subthreshold voltage imaging – we will develop a system with entirely new capabilities that will be broadly beneficial for neuroscience research and highly impactful for circuit and systems neuroscience. The system will combine high throughput single cell photo-stimulation with subthreshold postsynaptic membrane voltage recording to map and monitor and map synaptic weights across thousands of candidate synaptic connections. We intend to capitalize on this new platform to map and track synaptic weights in the visual cortex while an animal learns a visual perceptual task and during deprivation induced plasticity. In so doing, we aim to directly reveal the biological learning algorithms for experience dependent learning. Unlike all prior approaches in mammals which could only indirectly relate changes in neural activity to changes in synaptic connections with learning, our new approach will allow us to directly reveal how learning dynamically shapes synaptic weights in a neural network to give rise to altered neural representations that implement learning. Istituzione: UNIVERSITY OF CALIFORNIA BERKELEY PI: Hillel Adesnik Progetto: 1R01EY038065-01A1

Settori: National Eye Institute

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